Hands-on training in State-of-the-Art Methods in Stem Cells culture maintenance and differentiation #2

  • Location

    KFSH&RC

  • Phone

    +966 550422051

  • Website: No Website Join Event
  • Email Address: cme-r@kfshrc.edu.sa
  • Email Activity Coordinator: No data
  • Phone Activity Coordinator: No data

Event Audience

  • Technicians and Health Assistants
  • Biology ( Genomic & Biotechnology )

Event Goals

  • In a group, Participants will determine intracellular calcium homeostasis of iPSc.
  • Determine suitable type of protocol needed for cell harvesting.
  • Identify the parameters growth factor and lineage manipulation of differentiation for developing the needed cell type.
  • - Understand and allocate pluripotent cell state with light and fluorescence microscope and determine harvesting point.
  • Develop different cardiac, smooth muscle and epithelial cells from induced pluripotent stem cell mimicking human specific organ model

Event Agenda

22/06/2025

Introduction the training course structure-Introducing participants to each other, dividing participants into two groups of 5 (group A and B)- Participants will be given 2 experimental papers on stem cell

09:00:00 - 10:00:00
Speaker(s): ريم الحجيلان ( Reem AL Hejailan)
22/06/2025

Lecture about stem cells (types, pluripotency, signalling pathways, to read and discuss by the end of week 1. principles of differentiation etc)

10:00:00 - 11:00:00
Speaker(s): ريم الحجيلان ( Reem AL Hejailan)
22/06/2025

Touring the laboratory (Tissue culture and organ farm) and introducing participants to the practical sessions ( general rules like wearing lab coats and aseptic techniques using 70% ethanol etc

11:00:00 - 12:00:00
Speaker(s): ريم الحجيلان ( Reem AL Hejailan)
22/06/2025

Break

12:00:00 - 13:00:00
Speaker(s): No speakers assigned
22/06/2025

Guide before the star:f the practicals on stem cell culturing and charcterization of samples (markers

13:00:00 - 14:00:00
Speaker(s): ريم الحجيلان ( Reem AL Hejailan)
22/06/2025

Stem cell in culture

14:00:00 - 16:00:00
Speaker(s): ريم الحجيلان ( Reem AL Hejailan)
23/06/2025

reprograming of stem cells, quality control of stem cells and maintanance of stem cells.

09:00:00 - 10:00:00
Speaker(s): ريم الحجيلان ( Reem AL Hejailan)
23/06/2025

Stem Cell Harvesting

10:00:00 - 12:00:00
Speaker(s): ريم الحجيلان ( Reem AL Hejailan)
23/06/2025

Break

12:00:00 - 13:00:00
Speaker(s): ريم الحجيلان ( Reem AL Hejailan)
23/06/2025

Developmental bassis of differentiation into epithelial lung cells (ENDODERM)

13:00:00 - 14:00:00
Speaker(s): ريم الحجيلان ( Reem AL Hejailan)
23/06/2025

Stem cells alveolar differentiation Part I

14:00:00 - 16:00:00
Speaker(s): ريم الحجيلان ( Reem AL Hejailan)
24/06/2025

Developmental bassis of differentiation into Cardiac muscle cells (MESODERM)

09:00:00 - 10:00:00
Speaker(s): ريم الحجيلان ( Reem AL Hejailan)
24/06/2025

Stem cells Cardiac differentation Part I

10:00:00 - 12:00:00
Speaker(s): ريم الحجيلان ( Reem AL Hejailan)
24/06/2025

Break

12:00:00 - 13:00:00
Speaker(s): No speakers assigned
24/06/2025

Charachterization of alveiolar generated stem cells

13:00:00 - 16:00:00
Speaker(s): ريم الحجيلان ( Reem AL Hejailan)
25/06/2025

Stem cell based disease models and theraputic potential of stem Cells

09:00:00 - 10:00:00
Speaker(s): ريم الحجيلان ( Reem AL Hejailan)
25/06/2025

characterization of stem cells generated cardiac cells

10:00:00 - 12:00:00
Speaker(s): ريم الحجيلان ( Reem AL Hejailan)
25/06/2025

Break

12:00:00 - 13:00:00
Speaker(s): No speakers assigned
25/06/2025

Stem cells alveolar/cardiac differentiation Part II

13:00:00 - 16:00:00
Speaker(s): ريم الحجيلان ( Reem AL Hejailan)
26/06/2025

Intracellular calcium homeostasis in stem cells

09:00:00 - 10:00:00
Speaker(s): ريم الحجيلان ( Reem AL Hejailan)
26/06/2025

Intracellular calcium measurnment for stem cells Part 1han

10:00:00 - 12:00:00
Speaker(s): ريم الحجيلان ( Reem AL Hejailan)
26/06/2025

Break

12:00:00 - 13:00:00
Speaker(s): ريم الحجيلان ( Reem AL Hejailan)
26/06/2025

Intracellular calcium measurnment for stem cells Part 1

13:00:00 - 15:00:00
Speaker(s): ريم الحجيلان ( Reem AL Hejailan)
26/06/2025

Disscusion and closing remarks

15:00:00 - 16:00:00
Speaker(s): ريم الحجيلان ( Reem AL Hejailan)